Journal: Cellular and Molecular Gastroenterology and Hepatology
Article Title: A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium
doi: 10.1016/j.jcmgh.2019.02.010
Figure Lengend Snippet: H pylori infection induces increased chemokine expression in gastric tissues and enhances the recruitment of DCs to the gastric epithelium. ( A ) Heatmap showing relative chemokine gene expression in human gastric antrum and body from noninfected (Co) and H pylori –infected donors without atrophy (gastritis, Ga) and with gastric atrophy (Atr) (n = 3 each). Data were extracted from Gene Expression Omnibus data set records GDS5338 (antrum) and GDS5411 (body). Arrow indicates significant up-regulation (analysis of variance with the Dunnett multiple comparisons test, P ≤ .05). ( B–D ) Gastric biopsy samples from H pylori –infected (n = 11) and noninfected (n = 8) human donors were immunolabeled for HLA-DR (Cy3, red) and epithelial cytokeratin (type 7/8; FITC, green). Cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Images were acquired on a Nikon Eclipse T2000-U fluorescent microscope equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 software with a 20× objective. ( B ) Representative image of non– H pylori –infected mucosa. Arrows indicate examples of DCs in direct contact with epithelial cells. Scale bar : 20 μm. ( C ) The number of HLA-DR high DCs in direct contact with the basal side of the gastric epithelium was counted and normalized to epithelial length. Data from individual subjects, means ± SD, are shown. Data were analyzed by the Student t test. ( D ) The number of intraepithelial DCs was determined using the approach described for panel C .
Article Snippet: Samples were imaged on a Nikon Eclipse T2000-U fluorescent microscope (Nikon, Melville, NY) equipped with a CoolSnap ES digital camera and NIS Elements BR2.30 (Nikon).
Techniques: Infection, Expressing, Immunolabeling, Labeling, Microscopy, Software
